【原创】急救999

根据物质的特征看容易溶在什么溶剂里

人参皂苷 Are you talking about the Ginsenoside Rb1 or Gynosaponin C?
if yes, it 100% dissolve in MeOH.
标准品很贵: The price of Ginsenoside Rb1 is 10 mg/300$.

Here is my methods:

Luna C18, 150x4.6, 5um.
A-H2O, B-ACN: 10% B increase to 100% B over 30 min.
Temp:30 C.
Flow rate: 1.3 ml/min

Injection vol:10 ul
Injection Conc.:0.6 mg/ml in Methanol
Detector: 205 (+-) 4 nm.
Retension time: 8.2 min,
Mass Spec ( ESI+) : 969.64, 789.76,281.25, 207.14.
Mass Spec ( ESI-) : 945.62, 783.66,637.60,475.64.

Hope it works,
Let me know if want another assay Uv methods.
Best

你们不用药典方法吗

看了你的条件，10%的乙腈不能溶解吗？