pharmacy_detect 2009/04/29
荧光素钠可以用HPLC-DAD/ESI-MS来测定。荧光素具有香豆素类化合物基本母核,在UV 280nm有较强的特征吸收,可以设为UV检测波长。 提供一个参考色谱条件: column:C18 UV: 280 nm Mobile phase consisted of (A)water-acetonitrile-acetic acid (89:9:2) and (B) water-acetonitrile(20:80). The separation was performed using a linear gradient from 0% to 30% B in 30 min. The structural information was obtained using a Finnigan LCQ ion trap mass spectrometer (Thermoquest, San Jose, CA) equipped with an API chamber and an ESI source. The ionization mode was negative mode; 参考文献:Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe http://www.brunswicklabs.com/artman/uploads/jafc-boxin.pdf The potential of nasal application for delivery to the central brain—a microdialysis study of fluorescein in rats http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T25-4B8BXPN-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=154ecfe73b1d03475b3c61995700b263
zhufangwei 2009/04/21
[quote]原文由 [B]ada825[/B] 发表: 我实验中要测荧光素钠的浓度,本来是想用荧光检测器的HPLC测,但在仪器排期上遇到困难,现在可以有机会用液质,不知道这种物质是否可以用液质方法检测,有没有测过的,可以提供一下方法。如果可以测的话,是不是用液质的方法测在发表文章时会比HPLC更好一些?谢谢。[/quote] 看结构应该是可以用液质来做的,有必要才用液质做,没必要就没必要,如果单纯是研究的话,如果是做分析方法研究的话,液质应该比[url=https://insevent.instrument.com.cn/t/5p][color=#3333ff]液相[/color][/url]文章好发些。 [img]https://ng1.17img.cn/bbsfiles/images/2009/04/200904212014_145833_1644182_3.jpg[/img]
pharmacy_detect
第7楼2009/04/29
荧光素钠可以用HPLC-DAD/ESI-MS来测定。荧光素具有香豆素类化合物基本母核,在UV 280nm有较强的特征吸收,可以设为UV检测波长。
提供一个参考色谱条件:
column:C18
UV: 280 nm
Mobile phase consisted of (A)water-acetonitrile-acetic acid (89:9:2) and (B) water-acetonitrile(20:80). The separation was performed using a linear gradient from 0% to 30% B in 30 min. The structural
information was obtained using a Finnigan LCQ ion trap mass
spectrometer (Thermoquest, San Jose, CA) equipped with an
API chamber and an ESI source. The ionization mode was
negative mode;
参考文献:Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe
http://www.brunswicklabs.com/artman/uploads/jafc-boxin.pdf
The potential of nasal application for delivery to the central brain—a microdialysis study of fluorescein in rats
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T25-4B8BXPN-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=154ecfe73b1d03475b3c61995700b263