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【资料】Free-Zone Electrophoresis in Glass Capillaries

毛细管电泳(CE)

  • lucas 关于毛细管电泳的开创性的文献,是毛细管电泳技术走向商品化仪器的奠基文献。摘要见下:
    A simple theory of zone electrophoresis in open-tubular capillaries is developed. According to this theory, to achieve the highest resolution of zones, tubes with as small an inside diameter as possible should be used in combination with as high an applied voltage as feasible. To testthis approach, we performed electrophoresis In glass capillaries with an internal diameter of 75 m and a length of 100 cm. A special fluorescence detector was used to detect fluorescent zones while they migrated inside the capillary. With the application of 30 kV potentials to this system, rapid and efficient separations of amino acids,peptides, and urinary amines were demonstrated. In all cases fluorescent derivatives were necessary for detection. Preliminary results are encouraging, and with further development of sensitive detection devices, applications in clinical analyses may be feasible.
    clasic paper CZE LUCAS.PDF
  • 该帖子已被版主-sunpengwjh加3积分,加2经验;加分理由:鼓励分享!
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  • sunpengwjh

    第1楼2009/12/18

    不错的资料,谢谢楼主分享,楼主有没有关于手性整体住的文献?谢谢

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  • i_am_ling

    第2楼2009/12/18

    好文章感谢分享,斑竹想看整体住方面的,我想看手性拆分方面的文献,如果有的话,上传下,谢谢了!

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  • cpudaxiao

    第3楼2009/12/19

    我不做哪一块的,所以没有相关的文献。

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  • sunpengwjh

    第4楼2009/12/20

    楼主是做那个方向的?

    cpudaxiao(cpudaxiao) 发表:我不做哪一块的,所以没有相关的文献。

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  • cpudaxiao

    第5楼2009/12/20

    亲和毛细管电泳,老技术了!

    sunpengwjh(sunpengwjh) 发表:楼主是做那个方向的?

0
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  • i_am_ling

    第6楼2009/12/21

    不错的方向,已经有了一定的基础,也很成熟,不想电色谱,运行有些繁琐!

    cpudaxiao(cpudaxiao) 发表:亲和毛细管电泳,老技术了!

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  • 小雨哥

    第7楼2009/12/29

    来分享一下资料!楼主辛苦了啊!

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  • muziqing_0_0

    第8楼2010/04/18

    有蛋白方面的吗?植物的,我一直查不到新的

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