看这篇paper,首先要明白SEC-ICP-MS的分析目标是什么，简单来说，SEC是用来分离样品中分子量大小不同的蛋白质或者核酸的。和ICP-MS连用就可以在线分析不同的蛋白质上结合的各种金属元素含量。这篇paper里是分析多种奶样品中金属蛋白差异性，结果表明，人奶中金属与蛋白质键合情况与动物奶中不同。
把ICP-MS应用到biochem方面一直都是hot topics,可以发很多比较好的文章，不过用ICP-MS分析金属蛋白已经不算新方向了啊。
bluefox(freewbt) 发表:弱弱的问一下，最近看文献看到有研究元素的Fraction pattern，不理解是什么意思，请高手指教！
资料显示用尺寸排阻色谱ICP-MS测定牛奶乳清中Fe, Cu, Zn, Mn, Sr, I, Br, Ca and Mg 等元素的Fraction pattern，因没有下载到原文，所以具体不明白。
Multi-elemental fractionation in milk whey by size exclusion chromatography coupled on line to ICP-MS
Journal of analytical atomic spectrometry,2002, vol. 17, no10, pp. 1271-1277.
摘要：A fast hybrid technique based on HPLC coupled to ICP-MS was used to study elemental fractionation patterns in milk whey of different types of milk (human, cow and formula milk) taking advantage of the multi-elemental capability of ICP-MS. Milk whey was obtained from the corresponding whole milk samples by resorting to ultra-centrifugation. The method described separated the different fractions in a high-pressure column, based on size exclusion chromatography (SEC) on a TSK G 2000 SWXL column, coupled on-line to the inductively coupled plasma mass spectrometer for multi-elemental detection. After optimisation of the instrumental conditions, the method was applied to investigate the fractionation pattern of Fe, Cu, Zn, Mn, Sr, I, Br, Ca and Mg in four different samples of each kind of milk whey under study (human, raw cow, UHT cow and formula milk whey). As a result of this, it was found that the elemental binding patterns in human milk were different from those observed in cow and formula milks. This finding seems to indicate the presence in human milk of specific ligands. Thus, bioavailability of essential elements seems to be different in man. Therefore, more care and further study should be given to the characteristics of cow and formula milks used for human nutrition, particularly in babies and infants.
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