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【求助】请教:标准曲线问题!

  • kuuikaifeng
    2010/04/15
  • 私聊

液相色谱(LC)

  • 美国药典对一个有关物质的检测方法如下:
    Related compounds—
    Diluent, Solution A, Solution B, Peak identification solution, and Chromatographic system— Proceed as directed in the Assay.
    Standard solution— Dissolve an accurately weighed quantity of USP Capecitabine RS in Diluent, and sonicate if necessary, to obtain a solution having a known concentration of about 0.6 mg per mL
    Test solution— Dissolve an accurately weighed quantity of Capecitabine in Diluent, and sonicate if necessary, to obtain a solution having a known concentration of about 0.6 mg per mL
    Procedure— Separately inject equal volumes (about 10 µL) of the Standard solution and the Test solution into the chromatograph, record the chromatograms, and measure the peak responses. Calculate the percentage of each impurity in the portion of Capecitabine taken by the formula:
    100(1/F)(CS / CU)(rI / rS)
    请高手帮忙分析下,使用单点外标法吗?还是需要做标准曲线,谢谢!
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  • kuuikaifeng

    第1楼2010/04/15

    怎么没谁给说下??

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  • xiaojing2010

    第2楼2010/04/15

    是的,在标准曲线线性范围内才能用来计算。

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  • 有水有渝

    第3楼2010/04/15

    应助达人

    检查项目一般单点外标法就行

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