【求助】苍天呀，基线前面特别平，但快出峰时漂的特别厉害，这是怎么回事？

缓冲溶液用的什么？是用缓冲溶液溶解的样品么？

1. buffer capacity is not enough;
2. sample volume is too much;
3. there is particles in sample solution

这可能是温度和柱压变化造成的。与样品有关。

1. buffer capacity is not enough;
2. sample volume is too much;
I can't agree with you more,it used large volume injection,and buffer concentration is 20 mM borate buffer,

but 我前天做的基线就特别平，一点也不漂。

从新做实验了，那些溶液重新配置了？

主要是溶解样品的溶液极性与背景缓冲液的极性相差过大，或进样量过大。或者两者兼有

i agree that 溶解样品的溶液极性与背景缓冲液的极性相差过大

actually your work is acceptable - 基线前面特别平，出峰

thanks all the masters.