High-Performance Liquid Chromatography for the Determination of the Hippuric Acid as Urinary Metabolite of Toluene
Jie Liu, Yong Mei, YaNan Wang
College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China
Abstract
Objective:To establish a new analytical method for the determination of hippuric acid in human urine by HPLC. Method:Urine samples were collected at the end of the shift and analyzed for hippuric acid by HPLC with a uv/vis detector. A mixed solution of methanol/0.01mol/L phosphoric acid (40/60) as mobile phase was used. The detection was performed at λ=228nm, at 1.0ml/min. Results: The method showed good linearity in a range of 0.01mg/ml～0.5 mg/ml with a correlation coefficient of 0.9999. The average relative standard deviations(RSDs) at three concentration levels of low, medium and high were 2.59%, 0.90%, 0.68% respectively, and the average recovery were 92.25%, 97.27%, 100.15% respectively. The minimum detection concentration was 0.41mg/L. Conclusion:The developed method was simple, reliable, and useful for the biologic monitoring of industrial exposure to toluene with high reproducibility and stability.
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