如何用MALDI-TOF-MS进行蛋白样品预处理？

推荐你一个网址：
http://www.proteomics.com.cn/
是军事医学科学院国家生物医学分析中心的网址
里面有一个论坛，对你会有很多帮助。

Cylindrical gel samples of 1-mmdiameter were excised and destained by incubation with 400 uL
of 25% 2-propanol for 30 min. The destained gel samples were
dried in a Speedvac for 10 min, followed by addition of 5 uL of
digestion buffer (5 mM DTT, 5 mM n-octyl glucopyranoside (n-
OGP), 20 mM Tris, pH 7.8) containing 12 ng/ÌL modified porcine
trypsin (sequencing grade, Promega). After overnight incubation
at 37 °C, 5 ÌL of 0.4% TFA, 5 mM n-OGP was added and the
incubation was extended for 1 h at room temperature. To obtain
sufficient volume for comparison of sample preparation methods,
an additional 5 ÌL of 0.2% TFA, 5 mM n-OGP was added. Samples
were stored at -20 °C prior to MALDI sample preparation.

谢谢!

中关村附近除了化学所那里还有TOF？

北医有一台岛津公司的tof仪器，另外，北大生物系也有一台，有什么需要帮忙的吗？

我公司有一台，但不在北京。

蛋白样品，多少钱， 报个价

新推出的蛋白质分析质谱仪，分析最大质量达到10MDa，技术参数可浏览公司网页：http://www.comet.ch/index.php?id=21

北京质谱中心蛋白样品只测分子量250元,PMF350元.