DESTRUCTION OF ORGANIC MATTER IN PLANT AND ANIMAL TISSUE WITH PERCHLORIC ACID-NITRIC ACID MIXTURES
Publication: Handbook of INDUCTIVELY COUPLED PLASMA SPECTROMETRY Authors: M. Thompson and J N Walsh Field: Biology, Animal, Agriculture, plant
Reagents
(i) Nitric acid, ~ 70 % m/m (ii) Perchloric acid ~ 60 % m/m. (iii) Hydrochloric acid ( 6 M). Dilute hydrochloric acid ( 36 % m/m, 534 ml) to 1l. (iv) Calibration blank – hydrochloric acid ( 1 M).
Procedure
(i) Weigh each sample (2.00 g, dried at 105 °C and ground *) into a clean, dry, numbered conical flask. (ii) Add nitric acid (40 ml) to each flask, cover with a watch glass, and set aside in the fume cupboard Overnight. (iii) Place the flask on the hotplate and warm gently until frothing ceases and the solutions are almost clear. The nitric acid should be boiling gently ( but without major loss of volume) at the end of this stage. (iv) Allow the flasks to cool, and check that there are no fat globules floating in the liquid. (v) Add perchloric acid ( 3 ml), replace the flasks on the hotplace without cover glasses, and very gently heat until the contents have just reached dryness. Do not overheat. (vi) Allow the flasks to cool, and add water (2-3 ml) and hydrochloric acid (2.0 ml, 6 M) to each. Warm to dissolve the residues. (vii) Transfer the cooled solutions either to graduated test-tubes or to graduated flasks, and dilute to volume with water.
* Samples which are difficult to dry by heating (e.g soft fruits) can be freeze-dried, or homogenized and weighed wet. Moisture content can be determined separately.
植物和动物组织使用高氯酸和硝酸混合物消解
发表: ICP 手册 作者: M. Thompson and J N Walsh 应用领域: 生物学，动物学，农业，植物研究
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