Unfolding of Concanavalin A by the Trifluoroethanol

2015/09/17   下载量： 6 次

方案摘要

方案下载
应用领域 制药/生物制药
检测样本 预防类生物药品
检测项目 含量测定>蛋白质
参考标准 JIST

The Stopped Flow method is used to analyze reactions occurred during the duration of several milliseconds and several seconds meanwhile the CD measurement provides valuable information regarding the protein’s secondary structures and environment of aromatic amino acids. Therefore, CD measurement with Stoppedflow method provides one of the best techniques in analyzing the protein’s Unfolding and Refolding process. Concanavalin A (derived from Jack bean) in natural state has abundant β-sheet structures, however, it is known that its structure changes into the one with rich α-helix under trifluoroethanol (TFE) and its Unfolding process has been reported also. In this application, the model SFS-492 High-Speed Stopped-flow System was used to measure the Unfolding process of Concanavalin A under TFE. The Concanavalin A’s in pH2 hydrochloric acid gives a CD spectrum specific to β-sheet structure. In contrast, in a solution with 50% of TFE added it has a CD spectrum specific to α-helix structures. As seen in Figure 1, a big change from abundant β-sheet

方案下载
配置单
上一篇 HPLC 中的采样频率、响应时间和柱外效应
下一篇 CD的同时多探头SMP测量和使用积分球测量丙氨酸粉末样品

文献贡献者

佳士科商贸有限公司
银牌会员 丨 第10年
查看全部资料
相关仪器 更多
相关方案
更多

相关产品

当前位置： JASCO 方案 Unfolding of Concanavalin A by the Trifluoroethanol

关注

拨打电话

留言咨询