【分享】Mass Spectrometry: Instrumentation, Interpretation, and Applications 推荐好书

这本书不错，各位能学习一下最好

谢谢版主鼓励，再送上一本好书


Metabolome Analysis: An Introduction (Interscience Series on Mass Spectrometry)

By Silas G. Villas-Boas, Jens Nielsen, Jorn Smedsgaard, Michael A. E. Hansen, Ute Roessner-Tunali,

Publisher: Wiley-Interscience
Number Of Pages: 311
Publication Date: 2007-02-16
Sales Rank: 525611
ISBN / ASIN: 0471743445
EAN: 9780471743446
Binding: Hardcover
Manufacturer: Wiley-Interscience
Studio: Wiley-Interscience
Average Rating:

PREFACE.
LIST OF CONTRIBUTORS.
PART I: CONCEPTS AND METHODOLOGY.
1 Metabolomics in Functional Genomics and Systems Biology.
1.1 From genomic sequencing to functional genomics.
1.2 Systems biology and metabolic models.
1.3 Metabolomics.
1.4 Future perspectives.
2 The Chemical Challenge of the Metabolome.
2.1 Metabolites and metabolism.
2.2 The structural diversity of metabolites.
2.2.1 The chemical and physical properties.
2.2.2 Metabolite abundance.
2.2.3 Primary and secondary metabolism.
2.3 The number of metabolites in a biological system.
2.4 Controlling rates and levels.
2.4.1 Control by substrate level.
2.4.2 Feedback and feedforward control.
2.4.3 Control by “pathway independent” regulatory molecules.
2.4.4 Allosteric control.
2.4.5 Control by compartmentalization.
2.4.6 The dynamics of the metabolism—the mass flow.
2.4.7 Control by hormones.
2.5 Metabolic channelling or metabolons.
2.6 Metabolites are arranged in networks that are part of a cellular interactome.
3 Sampling and Sample Preparation.
3.1 Introduction.
3.2 Quenching—the first step.
3.2.1 Overview on metabolite turnover.
3.2.2 Different methods for quenching.
3.2.3 Quenching microbial and cell cultures.
3.2.4 Quenching plant and animal tissues.
3.3 Obtaining metabolites from biological samples.
3.3.1 Release of intracellular metabolites.
3.3.2 Structure of the cell envelopes—the main barrier to be broken.
3.3.3 Cell disruption methods.
3.3.4 Nonmechanical disruption of cell envelopes.
3.3.5 Mechanical disruption of cell envelopes.
3.4 Metabolites in the extra cellular medium.
3.4.1 Metabolites in solution.
3.4.2 Metabolites in the gas phase.
3.5 Improving detection via sample concentration.
4 Analytical Tools.
4.1 Introduction.
4.2 Choosing a methodology.
4.3 Starting point—samples.
4.4 Principles of chromatography.
4.4.1 Basics of chromatography.
4.4.2 The chromatogram and terms in chromatography.
4.5 Chromatographic systems.
4.5.1 Gas chromatography.
4.5.2 HPLC systems.
4.6 Mass spectrometry.
4.6.1 The mass spectrometer—an overview.
4.6.2 GC-MS—the EI ion source.
4.6.3 LC-MS—the ESI ion source.
4.6.4 Mass analyzer—the quadrupole.
4.6.5 Mass analyzer—the ion-trap.
4.6.6 Mass analyzer—the time-of-flight.
4.6.7 Detection and computing in MS.
4.7 The analytical work-flow.
4.7.1 Separation by chromatography.
4.7.2 Mass spectrometry.
4.7.3 General analytical considerations.
4.8 Data evaluation.
4.8.1 Structure of data.
4.8.2 The chromatographic separation.
4.8.3 Mass spectral data.
4.8.4 Exporting data for processing.
4.9 Beyond the core methods.
4.9.1 Developments in chromatography.
4.9.2 Capillary electrophoresis.
4.9.3 Tandem MS and advanced scanning techniques.
4.9.4 NMR spectrometry.
4.10 Further reading.


Metabolome Analysis: An Introduction

放不下了，分开发

5 Data Analysis.
5.1 Organizing the data.
5.2 Scales of measurement.
5.2.1 Qualitative data.
5.2.2 Quantitative data.
5.3 Data structures.
5.4 Pre-processing of data.
5.4.1 Calibration of data.
5.4.2 Combining profi le scans.
5.4.3 Filtering.
5.4.4 Centroid calculation.
5.4.5 Internal mass scale correction.
5.4.6 Binning.
5.4.7 Baseline correction.
5.4.8 Chromatographic profi le matching.
5.5 Deconvolution of spectroscopic data.
5.6 Data standardization (normalization).
5.7 Data transformations.
5.7.1 Principal component analysis.
5.7.2 Fisher discriminant analysis.
5.8 Similarities and distances between data.
5.8.1 Continuous functions.
5.8.2 Binary functions.
5.9 Clustering techniques.
5.9.1 Hierarchical clustering.
5.9.2 k-means clustering.
5.10 Classification techniques.
5.10.1 Decision theory.
5.10.2 k-nearest neighbour.
5.10.3 Tree-based classification.
5.11 Integrated tools for automation, libraries, and data evaluation.
PART II: CASE STUDIES AND REVIEWS.
6 Yeast Metabolomics: The Discovery of New Metabolic Pathways in Saccharomyces cerevisiae.
6.1 Introduction.
6.2 Brief description of the methodology used.
6.2.1 Sample preparation.
6.2.2 The analysis.
6.3 Early discoveries.
6.4 Yeast stress response gives evidence of alternative pathway for glyoxylate biosynthesis in S.
cerevisiae.
6.5 Biosynthesis of glyoxylate from glycine in S. cerevisiae.
6.5.1 Stable isotope labelling experiment to investigate glycine catabolism in S. cerevisiae.
6.5.2 Data leveraged for speculation.
7 Microbial Metabolomics: Rapid Sampling Techniques to Investigate Intracellular Metabolite
Dynamics—An Overview.
7.1 Introduction.
7.2 Starting with a simple sampling device proposed by Theobald et al. (1993).
7.3 An improved device reported by Lange et al. (2001).
7.4 Sampling tube device by Weuster-Botz (1997).
7.5 Fully automated device by Schaefer et al. (1999).
7.6 The stopped-flow technique by Buziol et al. (2002).
7.7 The Bioscope: a system for continuous-pulse experiments.
7.8 Conclusions and perspectives.
8 Plant Metabolomics.
8.1 Introduction.
8.2 History of plant metabolomics.
8.3 Plants, their metabolism and metabolomics.
8.3.1 Plant structures.
8.3.2 Plant metabolism.
8.4 Specific challenges in plant metabolomics.
8.4.1 Light dependency of plant metabolism.
8.4.2 Extraction of plant metabolites.
8.4.3 Many cell types in one tissue.
8.4.4 The dynamical range of plant metabolites.
8.4.5 Complexity of the plant metabolome.
8.4.6 Development of databases for metabolomics-derived data in plant science.
8.5 Applications of metabolomics approaches in plant research.
8.5.1 Phenotyping.
8.5.2 Functional genomics.
8.5.3 Fluxomics.
8.5.4 Metabolic trait analysis.
8.5.5 Systems biology.
8.6 Future perspectives.
9 Mass Profi ling of Fungal Extract from Penicillium Species.
9.1 Introduction.
9.2 Methodology for screening of fungi by DiMS.
9.2.1 Cultures.
9.2.2 Extraction.
9.2.3 Analysis by direct infusion mass spectrometry.
9.3 Discussion.
9.3.1 Initial data processing.
9.3.2 Metabolite prediction.
9.3.3 Chemical diversity and similarity.
9.4 Conclusion.
10 Metabolomics in Humans and Other Mammals.
10.1 Introduction.
10.2 A brief history of mammalian metabolomics.
10.3 Sample preparation for mammalian metabolomics studies.
10.3.1 Working with blood.
10.3.2 Working with urine.
10.3.3 Working with cerebrospinal fluid.
10.3.4 Working with cells and tissues.
10.4 Sample analysis.
10.4.1 GC-MS analysis of urine, plasma, and CSF.
10.4.2 LC-MS analysis of urine, blood, and CFS.
10.4.3 NMR analysis of CSF, urine, and blood.
10.5 Applications.
10.5.1 Identification and classification of metabolic disorders.
10.6 Future outlook.
INDEX.

虽然俺发怵英语，但是既然这么好的书，俺就啃啃吧
谢谢分享

应助达人

这本：Metabolome Analysis: An Introduction (Wiley - Interscience Series on Mass Spectrometry) (Hardcover)
在美国亚马逊网上书店优惠价是：69美元，当当网价559元。
看翻译叫：相位谱分析导论

意思是用质谱做代谢组分析。采用液相色谱-质谱联用（LC-MS）的方法，比较不同血样中各自的代谢产物以确定其中所有的代谢产物---代谢物指纹分析（代谢组学的研究方法之一）。

仪器刚来，我要好好看看，学学准备开展这个项目!

为鼓励版友积极分享自己的电子书，特为wangs版友加精。

应助达人

如果有中文版的多好啊！！！

英文的？
估计看完都猴年马月了