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【分享】Pulse Program for Bruker Avance Spectrometers

核磁共振技术(NMR)

  • Pulse Program Library for Bruker Avance Spectrometers
    可进入下面网页在线阅读,粘贴下载。
    http://www.nmrfam.wisc.edu/~abild/Pulseprograms/index.html

    About this NMR pulse program library

    The main emphasis of this pulse program library is directed towards protein NMR spectroscopy of isotope enriched proteins in solution.

    All pulse programs are written for the use on Bruker Avance consoles running Xwin-NMR version 2.x. Most sequences will work with Xwin-NMR version 1.3 as well.
    The rf-channel assignments used are: 1H on f1, 15N on f2, 13C on f3. This may be changed by simply changing the define statement at the top of the pulse program.
    例子:
    2D 15N-FHSQC, with optional 13C decoupling and SEDUCE-1 decoupling


    2D 15N-1H Fast HSQC with minimal water saturation. Use optional 13C decoupling and SEDUCE-1 decoupling with 13C labeled samples. This version uses the gp style gradients.

    References
    Mori et al., J. Magn. Reson. B 108, 94-98 (1995)

    Sklenar, Piotto, Leppik, and Saudek: J. Magn. Reson. A 102, 241-245 (1993)

    Acquisition
    Pulse program
    fhsqc.fa

    Include files etc.
    Include file for optional expt time correction: faexptcorr.incl

    Composite pulse decoupling program for SEDUCE-1 decoupling: waltz16sp3

    Shaped pulse file for optional compensated SEDUCE-1 decoupling: seduce1c6.jc (JCAMP-DX), seduce1c6.rf (binary RF format) or seduce1c6.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 6*(1/P90) Hz from carrier)

    Shaped pulse file for optional compensated SEDUCE-1 decoupling: seduce1c5.jc (JCAMP-DX), seduce1c5.rf (binary RF format) or seduce1c5.txt (ASCII) (cosine modulated SEDUCE-1 shape with excitation optimum at 5*(1/P90) Hz from carrier)

    Read more about the pulse shape file formats.

    Parameters
    Acquisition parameters: 500 MHz, 750 MHz

    Processing
    Use ux2flx or equivalent utility to convert your Bruker data to Felix "dat" format.

    Felix95 processing macro: ft2d_fhsqc.mac

    Also used (for correction of digital filter artifacts): digphs.mac




    ---------------------------------------------------------



    Table of contents
    About this pulse program library


    How to use the files from this library


    Legend

    T : TROSY
    C : CryoProbe ready




    2D Heteronuclear Shift Correlation
    fhsqc.fa
    n15hsqcse.fa
    cthsqcse.fa
    c12cosyc12.fa
    trosy.fa T C
    trosysefb.fa T C
    c13cttrosy.fa T


    3D/4D Heteronuclear Shift Correlation
    hncatrkay.fa T
    hncotrk2b.fa T C
    hncacbtr.fa T
    hncocacbtr.fa T
    hncacotrb.fa T C
    hncocatr.fa T
    n15tochsqcse.fa
    n15tocfhsqc.fa C
    toccthsqcse.fa
    hncof.fa C
    hncose.fa
    hncaf.fa
    hncocaf.fa C
    hncacbf.fa
    hcchtocsy.fa
    hcchcosy.fa
    hcconhse.fa C
    cconhse.fa C
    hncacof.fa
    hncocacbf.fa C
    hcacose.fa
    hbacbanhf.fa
    hbcbconhf.fa
    cbcaconhse.fa
    toccthsqcse.fa
    cdcancocaha.fa C


    2D/3D/4D Homonuclear Exchange Spectroscopy - Isotope Enhanced
    noetrosysefb.fa T
    noetrosyfb.fa T
    n15noefhsqc.fa C
    c13noehsqc.fa
    c13noe3d.fa
    cnnoesy4d.fa
    ccnoesy4d.fa
    noehncof.fa
    noecthsqcse.fa
    hcchnoesy.fa


    Relaxation Time Measurement
    n15t1.fa
    n15t2.fa
    hn15noegp.fa
    hn15noe.fa


    2D Homonuclear Exchange Spectroscopy
    noesy3919.fa
    roesy3919.fa


    2D Homonuclear Shift Correlation
    tocsy3919.fa


    Calibration Sequences
    zgsel1.fa
    zgsel2.fa
    zgsel3.fa




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