【求助】注射液用高效液相怎么检测？？

liuqiasnqia

2011/05/24

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液相色谱（LC）

土霉素注射液用高效液相怎么检测？谢谢！

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kylabs

第1楼2011/05/24

注射用土霉素的稳定性分析方法：

Column

Cogent Bidentate C18™, 4µm, 100A

Catalog No.

40018-75P

Dimensions

4.6 x 75 mm

Solvents

A: DI water + 0.1% formic acid
B: acetonitrile + 0.1% formic acid

Gradient

time (min.)	%B	time (min.)	%B

0	10	6	80
4	30	7	10

Post time

3 min.

Flow rate

1.0 mL/min.

Sample

USP grade tetracycline HCl capsule extract (degraded).
Stock Solution: 10.0 mg capsule contents were diluted with 10 mL 1.0 N HCl and sonicated for 10 min. 1 mL aliquot was diluted to 20 mL with DI water. Solution was heated at 80°C for 30 min. Solution was then diluted to 100 mL with DI water and filtered through a 0.45 µm nylon membrane HPLC filter (MicroSolv Technology Corp., Eatontown, NJ, USA).
Working Solution: Stock solution was diluted 10x using 0.01 N HCl diluent.

Detection

UV 360 nm (0? min) 430 nm (5? min)