Do not use any of the following solvents in the Waters Reagent Manager because they damage PEEK components. • Methylene chloride • Tetrahydrofuran (THF) at greater than 10% (v/v) • Freon • Concentrated sulfuric acid (H2SO4) • Concentrated nitric acid (HNO3) Also keep in mind the following solvent guidelines: • Use methanol to prime a new pump or a pump that has been stored for an extended period. • Use an intermediate solvent when you change between solvents that are not miscible. • Always keep the solvent level in the reservoir at least 4 inches ( 10cm) higher than the pump inlet. Do not place solvent reservoirs on top of the Reagent Manager. • Use only filtered, degassed solvents. • After using a buffer, purge the pump with HPLC-grade, distilled, or deionized water to prevent crystallization and damage to the piston or seal. • Know the properties of the solvents under different pressures. • Dedicate a solvent supply tube and filter assembly for each solvent. If this is not possible, purge the tube and filter of any remaining solvent before you use a new solvent. • To prevent cross-contamination of solvents, dedicate a set of glassware for preparing, storing, and supplying each solvent. Once a container has been used for a solvent, do not use it for any other solvent unless it has been thoroughly cleaned.
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