【资料】Microarray protocols(转贴)

Fast Track mRNA Isolation (Brown Laboratory ­ Stanford)


Fast Track mRNA Isolation (DeRisi Laboratory ­ UCSF)


Human Tumor mRNA Isolation (DeRisi Laboratory ­ UCSF)


Preparation of Total RNA from Blood (Brown Laboratory-Stanford)


RNA Extraction (NHGRI)


Poly A+ Isolation from Total RNA (TIGR)


RNA Isolation (Microarray Core Services - University of Rochester)

RNA Isolation (Commercial Kits)
FastTrack 2.0 mRNA Isolation Kit (Invitrogen)

Micro-FastTrack 2.0 mRNA Isolation Kit (Invitrogen)


mRNA Catcher (Invitrogen)


TRIzol Reagents (Invitrogen)


RNA Stabilization and Purification from Animal, Plant, Bacterial, Yeast, and Fungal Samples (Qiagen)

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QIAquick PCR Purification Kit (Qiagen)
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Technical Article (Ambion, Inc.)


Total RNA isolation kits and reagents (Ambion, Inc.)


mRNA isolation kits and reagents (Ambion, Inc.)


ArrayIt Micro-Total RNA Extraction Kit (Telechem)

RNA Amplification (Academia/Non-profit)

aRNA Amplification - Ambion Method (Brown Laboratory ­ Stanford)


aRNA Amplification - Wang Method (Brown Laboratory ­ Stanford)


aRNA Amplification (Brown Laboratory ­ Stanford)


Modified Eberwine RNA Amplification

RNA Amplification (Comercial Kits)

Amino Allyl MessageAmp aRNA Kit (Ambion, Inc.)


MessageAmp II aRNA Amplification Kit (Ambion, Inc.)


BDSmart mRNA Amplfication Kit (BD Biosciences Clontech)


Ovation System (Nugen)


ArrayIt MiniAmp mRNA Amplification Kit (Telechem)

Probe Labeling (Glass Slide Arrays - Academia/Non-profit)

Labeling Yeast RNA (Brown Laboratory ­ Stanford)


Labeling Human RNA (Brown Laboratory-Stanford)


Labeling E.Coli RNA (Brown Laboratory-Stanford)


Amino-Allyl Reverse Transcription (Brown Laboratory-Stanford)


Revised Amino-Allyl Labeling (Brown Laboratory-Stanford)


Labeling by Reverse Transcription (NHGRI)


Amino-allyl Dye Coupling Protocol


Reverse Transcription/Labeling RNA


Modified Eberwine RNA Amplification


Amino Allyl Labeling (TIGR)


Direct Probe Labeling (University of Queensland)

Probe Labeling (Commercial Kits)

LabelStar Array Kit (Qiagen)


Amino Allyl cDNA Labeling Kit (Ambion, Inc.)


FairPlay® II Microarray Labeling Kit (Stratagene)


LabelStar Array Kit (Qiagen)


Ovation Aminoallyl System (Nugen)


ArrayIt Indirect Amino Allyl Fluorescent Labeling Kit (Telechem)


ULS™ labeling kits for DNA microarray applications (Kreatech)


Microarray Target Purification Kit (Roche Biochemicals)


Label IT® µArray™ Cy™3/Cy™5 Labeling Kit (Mirus Bio Corp.)

Hybridization & Washing (Glass Slide Arrays)

Array Hybridization-General/Yeast (Brown Laboratory-Stanford)


Array Hybridization-Human (Brown Laboratory-Stanford)


Array Hybridization (DeRisi Laboratory ­ UCSF)


Array Washing (DeRisi Laboratory ­ UCSF)


Hybridization (NHGRI)


Labeled Probe Hybridization (TIGR)


Hybridization/Washing Glass Slide Arrays (QUANTIFOIL)


Array Washing Protocol (QUANTIFOIL)

Scanning (Glass Slide Arrays)

Array Scanning (DeRisi Laboratory ­ UCSF)

Probe Labeling (Filter Arrays - Academia/Non-profit)

Showe Lab (The Wistar Institute)


OU Microarray Core Facility (University of Oklahoma)


Saied A. Jaradat, Ph.D., Tetsuya S. Tanaka, Ph.D. and Yulan Piao, M.D ­ (National Institute of Aging)

Affymetrix GeneChips® Protocols

BioMolecular Resource Facility (Australian National University)


W.M. Keck Facility (Yale University)

辛苦了，斑竹！
收集了这么全的资料，要好好学习一下啊！