Single Fiber Analysis Acrylic fibers containing unknown dyes were used for testing the optimized parameters for basic dyes. A standard 1:1 formic acid and water (13) solution was utilized as the extraction solvent system. A single fiber of 0.5 cm was placed in a vial insert and extracted. This vial was then placed in the autosampler where 10 μL injections were placed on column and analyzed using the basic dye-optimized parameters. The disperse dye method was also evaluated using single fiber samples. Polyester fabric swatches were dyed with disperse dyestuffs, and 0.5 cm single fibers were extracted using the acetonitrile and water system. The fibers were placed in vial inserts and extracted with 50 μL of solvent at 120°C for 60 min. The fibers were then analyzed using the optimized parameters for the disperse dye method. Chromatography Optimization The final optimized parameters for the basic dyes can be seen in Table 2 and the chromatogram of the basic dye mix can be seen in Fig. 1. Of the 15 dyes in the mix, 11 were able to be resolved using the gradient that was developed. The other four dyes (BY28, BBlk, BB77, and BB45) could not be resolved by adjusting the HPLC parameters. FIG. 1—Chromatogram of separated basic dyes.
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