一本关于HPLC的使用指南（英文原版电子书） 书名：HPLC：A Practical User’s Guide 主编：Marvin C. McMaster 版本：SECOND EDITION 出版社：A John Wiley & Sons 目录： PREFACE xi I HPLC PRIMER 1 1 Advantages and Disadvantages of HPLC 3 2 Selecting an HPLC System 15 3 Running Your Chromatograph 25 II HPLC OPTIMIZATION 43 4 Separation Models 45 5 Column Preparation 61 6 Column Aging, Diagnosis, and Healing 73 7 Partition Chromatography Modifications 89 8 “Nonpartition” Chromatography 95 9 Hardware Specifics 105 10 Troubleshooting and Optimization 125 III HPLC UTILIZATION 135 11 Preparative Chromatography 137 12 Sample Preparation and Methods Development 143 13 Application Logics: Separations Overview 159 14 Automation 167 15 Recent Advances in LC/MS Separations 181 16 New Directions in HPLC 195 APPENDICES 201 APPENDIX A Personal Separations Guide 203 APPENDIX B FAQs for HPLC Systems and Columns 205 APPENDIX C Tables of Solvents and Volatile Buffers 211 APPENDIX D Glossary of HPLC Terms 213 APPENDIX E HPLC Troubleshooting Quick Reference 221 APPENDIX F HPLC Laboratory Experiments 227 Laboratory 1—System Start-up and Column Quality Control / 227 Laboratory 2—Sample Preparation and Methods Development / 229 Laboratory 3—Column and Solvent Switching and Pacification / 231 Appendix G Selected Reference List 233 INDEX 235
HPLC：A Practical User’s Guide
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