HPLC在肽类及蛋白类物质分析中应用（原版电子书） 书名：HPLC of Peptides and Proteins：Methods and Protocols 主编：Marie-Isabel Aguilar 出版社：Humana Press 时间：2004 目录： PART I MODES OF HPLC 1 HPLC of Peptides and Proteins: Basic Theory and Methodology ................................................ 3 2 Reversed-Phase High-Performance Liquid Chromatography ..... 9 3 Ion-Exchange Chromatography .................................................. 23 4 High-Performance Hydrophobic Interaction Chromatography... 45 5 Gel Filtration Chromatography .................................................... 55 6 Hydrophilic Interaction Chromatography .................................... 75 7 Immobilized Metal Ion Affinity Chromatography of Proteins ...... 89 8 Immunoaffinity Chromatography of Proteins ............................ 103 9 Liquid Chromatography–Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins ...................... 111 PART II PREPARATIVE METHODOLOGIES 10 Capillary Separations ................................................................ 143 11 Micropreparative HPLC of Peptides and Proteins ................... 165 12 Multidimensional HPLC Purification of Proteins ....................... 177 13 Analytical High-Performance Liquid Chromatography ............. 183 14 Prep/Semiprep Separations of Peptides .................................. 191 15 Large-Scale Protein Chromatography ...................................... 211 PART III APPLICATIONS 16 Proteolytic Peptide Mapping ..................................................... 227 17 Mass Spectrometric Characterization of Posttranslationally Modified Proteins—Phosphorylation..................................... 245 18 Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography–Mass Spectrometry and Liquid Chromatography–Tandem Mass Spectrometry .... 263 19 HPLC in the Analysis of Peptide Metabolism ........................... 275 20 Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells .................................................. 291 21 Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques .................................... 307 22 HPLC and Mass Spectrometry of Intrinsic Membrane Proteins .................................................................................. 323 23 IgG Purification .......................................................................... 341 24 DNA–Binding Proteins: LC–MS to Identify Key Domains in RNA Polymerase–Promoter Interactions .......................... 351 25 Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC ........................................................................... 365 26 Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry ........................................................... 377 27 Proteome Analysis .................................................................... 387 Index ................................................................................................. 401
HPLC of Peptides and Proteins：Methods and Protocols
迎国庆送壕礼！十月发帖抢大疆云台
【官方邀请】高效液相色谱使用情况有奖调研
喜迎国庆！第十七届原创大赛超级原创日参赛奖励翻倍
丹纳赫四十周年寄语征集领大礼包
