井冈霉素A的反相高效液相色谱分析
马婧玮1，陈 黎1 ，蒋格平2 ，潘灿平1
(1.中国农业大学 理学院，北京 100094； 2.浙江省义乌市环保局，浙江 义乌 322000)
摘要：建立了一种用反相高效液相色谱测定井冈霉素A的定量分析方法。使用新型Venusil HILIC液相色谱柱，以乙腈与水不同比例进行梯度洗脱，流速1.0 mL/min，紫外检测波长为210 nm。 该方法的标准偏差为0.24，变异系数为0.42%，回收率在96.5%~101.8%之间，线性相关系数为0.9987。 该方法简便、快速、准确、精密，适用于井冈霉素A 的定量分析。
关键词：井冈霉素A ；高效液相色谱分析；分析方法
中图分类号：TQ450.7 文献标志码：A 文章编号：1006-0413(2007)10-0686-02
Determination of Jingangmycin A by Reversed-phase HPLC
MA Jing-wei1, CHEN Li1, JIANG Ge-ping2, PAN Can-ping1
(1.College of Sciences, China Agricultural University, Beijing 100094, China; 2.Agency of Environmental Protection, Yiwu City, Zhejiang Province, Yiwu 322000, Zhejiang, China)
Abstract: A method for quantitative analysis of fungicide jingangmycin A was developed by areversed-phase high performance liquid chromatograph on a Venusil HILIC column by a lineargradient elution of different ratio of acetonitrile/water by volume as mobile phase with 1.0 mL/min flow ratio and UV detection at 210 nm. The standard derivation was 0.24, coefficient of variation was 0.42%, recovery was 96.5 to 101.8%, and the liner correlation coefficient was 0.9987. The method was proved to be simple, quick, accurate and precise to quantitative measure for jingangmycin A.
Key words: jingangmycin A; high performance liquid chromatography analysis; analysis method
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