本法用甲醇-水为流动相,在C18反相柱上建立了陆英中具有乌索酸含量测定的高效液相色谱法,以95%乙醇为溶剂处理样品具有提取率高、色谱干扰小、物质分离效果好的优点,本法简便易行、快速准确,其最小检出限为0.2ug。不同浓度水平检测结果日间、日内相对误差小于4.0%。 关键词:陆英草 乌索酸 高效液相色谱 含量测定 Analysis of Ursolic Acid in Herba Sambuci Chinensis using Hplc The paper reported the determination of Ursolic Acid inHerba Sambuci Chinensis using Hplc,the separation was achieved by applying an Waters -ODS 150×4.6 mm (5um) column and methanol-water (90:10) as mobile phase at flow rate of 0.8 ml/min. the UV detector was set at 210nm External standard method was applied .The linear range was 150-2000ug/ml with the lower limit of detection of 0.2ug.It was found to be effielent and low interference to extract the samples with ether.