This is Daily English Learning of Analytical Instrument. Today the topic is High-Performance Liquid Chromatography（HPLC） NOW let's begin the part 1 Vocabularies: 自动进样器 automatic sampler 高效柱 high performance column 高压输液泵 high pressure pump 荧光检测器 fluorescence detector 紫外-可见光检测器 ultraviolet visible detector 噪信比 noise -signal ratio 外标法 external standard method 峰高 peak height 保留时间 retention time 程序升温进样 programmed temperature sampling 基线 baseline 二 Sentence: 1. 应用高效液相色谱技术优化了酰氯化反应时间。 The chloridization time is optimized with high performance liquid chromatography. 2. 本文用高效液相色谱法对骨碎补配方颗粒的进行质量研究。 In this paper, high-performance liquid chromatography method is used on dispensing granules of Rhizoma Drynariae for qualitative research. 3. 产品经元素分析、高效液相色谱分析证实了它的结构和纯度。 The constitution and purity were confirmed by element analysis and HPLC detection. 4. 建立了高效液相色谱法检测植物性原料中三聚氰胺的方法。 An analytical method for determination for melamine in vegetable materials by high performance liquid chromatography was developed. 5. 对高效液相色谱条件进行优化，建立了分析方法，并用于实际水样分析。 The determination condition for HPLC was optimized and applied for practical water sample analysis. 三 Principles:
High- performance liquid chromatography is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column. The schematic of an HPLC instrument typically includes a sampler, pumps, and a detector. The sampler brings the sample mixture into the mobile phase stream which carries it into the column. The pumps deliver the desired flow and composition of the mobile phase through the column. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor and user software control the HPLC instrument and provide data analysis.
仪器信息网“仪友会”招募令
科学仪器品牌联合“仪器心得”征文活动
【生活中的仪器检测】有奖征文
LC-MS实验瓶颈的突破与优化
