【求助】雷帕霉素的处理

如果浓度太低，估计会检测不出来的。我没做过。样品稳定性好的话，可以用有机溶剂萃取，浓缩。不稳定，就用固相萃取小柱附集。如果是碱性，可以选用酸性柱。

此药在水中,缓冲液中也是溶解的,应该是属于抗生素药物.用有机相萃取能萃取出来么?

我现在想萃取,想浓缩,请教大家方法,我该怎么做?

可以考虑使用二氯甲烷萃取，40度下真空浓缩。

Streptomyces hygroscopicus
RAPA, Rapamune, Sirolimus, RPM, Antibiotic AY 22989
Rapamycin (Sirolimus) is a macrolide compound that acts by selectively blocking the transcriptional activation of cytokines thereby inhibiting cytokine production. It is bioactive only when bound to IMMUNOPHILINS. Sirolimus (Rapamycin) is a potent immunosuppressant. Sirolimus (Rapamycin) possesses both antifungal and antineoplastic properties.
53123-88-9
12, 8288
914.2

C51H79NO13

Specifications   
Appearance:     Off-white to slight yellow powder
Purity    At least 97% by TLC, HPLC
max:    265, 277, 287
Melting point    178°C-182°C
Solubility    Rapamycin (Sirolimus) gives clear colorless solution at 50mg/ml DMSO
Storage    -20°C. Protect from light and moisture. Hygroscopic.