paeonia0309
第2楼2009/04/17
1.你们仪器的校验周期是多长时间啊?
这个要根据你使用仪器的频次,一般性使用一年吧
2.在仪器校验的过程中,如果没有流量计,流速你们一般用什么方法测定?
没有流量计,方法同楼上说的,但是不是用量筒,使用容量瓶,精度更好
3.检测器的波长准确度用什么方法校验?
波长准确度可以选用咖啡因标准液
例如:
WAVELENGTH ACCURACY
If there are built-in test procedures for the determination and adjustment of wavelength accuracy, follow the instructions of the instrument manual.In all other cases use the procedure described below.
Solutions:
DAD: caffeine Std. 5 from the linearity testing UV/VIS: 1.0 µg/ml anthracene solution in water
Settings:
Mobile phase: 15% acetonitrile in water Column: RP18, 5 µm 30-50 x 2,1-4.6 mm or capillary 2.0 m x 0.12 mm ID Oven temperature: 40 °C Flow rate: 1.0 ml/min (adjusted by using 15% acetonitrile in water) Detection: scan from 230 nm to 290 nm (DAD) Injection volume: 20 µl
Method:
DAD: Inject 20 µl of the caffeine solution and record the spectrum. The maximum is at 272 nm and the minimum at 244 nm.
UV/VIS: fill the cell with the anthracene solution and change the wavelength from 248 to 254 nm in 1 nm steps; record the maximum of absorption. The theoretical value is 251 nm.
Limits: ±2 nm
有水有渝
第9楼2009/04/18
[url=https://insevent.instrument.com.cn/t/5p]液相色谱仪检定规程JJG 705-2002[/url]