【求助】UPLC和HPLC的区别在什么地方？用超高效要注意什么？

应助达人

区别在于高压，原理都一样
最要注意溶液要过滤，否则最容易堵，会造成一系列的后果

应助达人

Waters的UPLC处于领跑地位，不过也相对比较贵。

如果配二极管阵列检测器大概的多钱？

应助达人

在提醒一点，容易过载，我们用习惯了普通液相，以习惯性思维去想时，经常出现样品过载

Compare to HPLC, UPLC provide high resolution, high separation effciency, since the BEH column with 1.7um particle size, for the conventional mode of hplc, the particle size mostly around 5um and the UPLC pump can stand with 15000psi, the peak capacity is higher and resolving power is much better.
then most important is the buffer used and the sample should be cleaned as possible to prevent blocking the column and damage for the pump

也想学习一下

Waters的比较好，但是比较贵，通常一般不需要配UPLC，比较较贵，容易堵，而且其他的配件都贵

应助达人

叫法不同 都一样^_^

ding.........................