【求助】求6-甲基尿嘧啶的LC方法

Method Conditions



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Column Cogent Diamond Hydride™, 4µm, 100A
Catalog No. 70000-75P
Dimensions 4.6 x 75 mm
Mobile Phase DI Water + 0.1% Acetic Acid
Flow rate 1.0 mL/min.
Sample 1. Uracil
2. Guanine
3. Thymine
4. Adenine
Injection Volume 2.5 µL
Detection UV 254 nm
Temp 25° C


Discussion

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This method is easy to prepare, use and reproduce. Note Excellent separation under 100% aqueous conditions with alternate selectivity. These bases are difficult to retain on columns with ordinary silica that contain significant amounts of silanols.