仪器信息网APP
选仪器、听讲座、看资讯

Cell Stem Cell:日成功培育出无免疫力猪

  • zhang8826857
    2012/07/08
  • 私聊

生命科学仪器综合讨论

  • 6月13日,美国科技期刊Cell Stem Cell上发表的一篇研究论文称,日本农业生物资源研究所研究小组成功培育出无免疫力猪。这是世界首次人工培育成功无免疫机能的大型动物。

    研究小组提取猪胎儿体细胞,导入无免疫机能基因,利用体细胞克隆技术,克隆出40头遗传基因相同的猪仔,其中14头没有免疫机能所必须的胸腺。由于免疫机能丧失,易患感染症等,14头中只有5头顺利接受了正常猪的骨髓移植,其中3头目前已存活1年零2个月以上。

    目前,有关白血病及新药开发等的研究多利用免疫机能不全的老鼠,移植入人体细胞和组织等,观察病理反应和药效。但老鼠脏器与人体相差较大,寿命仅有2-3年,不能满足对疾病和药效的长期观察、验证需要。寿命达10年以上无免疫机能大型动物的培育成功,为iPS细胞安全性确认、人体器官再生以及新药开发等提供了更加有利的科研手段
    +关注 私聊
  • zhang8826857

    第1楼2012/07/08

    Il2rg Gene-Targeted Severe Combined Immunodeficiency Pigs

    Shunichi Suzuki, Masaki Iwamoto, Yoriko Saito, Daiichiro Fuchimoto, Shoichiro Sembon, Misae Suzuki, Satoshi Mikawa, Michiko Hashimoto, Yuki Aoki, Yuho Najima, Shinsuke Takagi, Nahoko Suzuki, Emi Suzuki, Masanori Kubo, Jun Mimuro, Yuji Kashiwakura, Seiji Madoiwa, Yoichi Sakata, Anthony C.F. Perry, Fumihiko Ishikawa, Akira Onis

    A porcine model of severe combined immunodeficiency (SCID) promises to facilitate human cancer studies, the humanization of tissue for xenotransplantation, and the evaluation of stem cells for clinical therapy, but SCID pigs have not been described. We report here the generation and preliminary evaluation of a porcine SCID model. Fibroblasts containing a targeted disruption of the X-linked interleukin-2 receptor gamma chain gene, Il2rg, were used as donors to generate cloned pigs by serial nuclear transfer. Germline transmission of the Il2rg deletion produced healthy Il2rg+/? females, while Il2rg?/Y males were athymic and exhibited markedly impaired immunoglobulin and T and NK cell production, robustly recapitulating human SCID. Following allogeneic bone marrow transplantation, donor cells stably integrated in Il2rg?/Y heterozygotes and reconstituted the Il2rg?/Y lymphoid lineage. The SCID pigs described here represent a step toward the comprehensive evaluation of preclinical cellular regenerative strategies.

0
猜你喜欢最新推荐热门推荐更多推荐
举报帖子

执行举报

点赞用户
好友列表
加载中...
正在为您切换请稍后...