maolg 2007/04/18
一个很好的适合液质、[url=https://insevent.instrument.com.cn/t/bp][color=#3333ff]气质[/color][/url]的前处理方法 [img]https://www.instrument.com.cn/bbs/images/affix.gif[/img][url=http://www.instrument.com.cn/bbs/download.asp?ID=49070]ppt[/url]
瓢虫 2007/04/23
推荐一本书,"Mass Spectrometry in Drug Discovery",里面对液质联用有详细的介绍,从液质联用在药物研发中的应用,质谱的离子源,如何做液质联用,化合物在质谱中的断裂机理,样品前处理,液质联用在药代、组合化学、体外样品分析中的应用都有详细的介绍,这本书是几个大药厂的作者写的,很实用! "Mass Spectrometry in Drug Discovery" edited by David T. Rossi & Michael W. Sinz (2002) ISBN 0-8247060-7-2. This state-of-the-art reference summarizes the theory, instrumentation, and application of mass spectrometry and atmospheric pressure ionization to screen, evaluate, and improve the performance and quality of drug candidates梠ffering current techniques in pharmaceutics and pharmacokinetics for increased quantities of new pharmaceutical compounds. Providing time- and cost-efficient approaches for the generation and analysis of effective pharmaceuticals, Mass Spectrometry in Drug Discovery covers o recent advances in combinatorial chemistry, molecular biology, bioanalysis automation, and computing o cassette dosing in drug discovery o the utilization of liquid chromatography/mass spectrometry (LC/MS) in chemical library characterization, combinatorial chemistry, drug transport, and in vivo microdialysis o reliable methods of sample preparation and handling o novel strategies to determine and characterize metabolite structures o the impact of absorption, distribution, metabolism, and elimination properties on drug discovery o the latest research using stable-labeled compounds Demonstrating the use of mass spectrometry in the assessment of disease states, drug targets, and potential drug agents, Mass Spectrometry in Drug Discovery is an invaluable source for spectrometrists; pharmacologists; analytical, medicinal, and organic chemists and biochemists; biotechnologists; pharmaceutical scientists; toxicologists; and upper-level undergraduate and graduate students in these disciplines. 结合书上看的和个人的理解讲讲液质联用的前处理(主要针对药物方面的)。 主要的预处理方法有: 1,直接稀释(适合基质简单的样品,如制剂样品) 2,蛋白沉淀(适合极性较强的化合物,一般用甲醇或乙腈作为沉淀剂) 3,液液萃取(适合大部分化合物,可以选择一系列的提取试剂,极性强的一般选用:乙酸乙酯,非极性强的一般选用:叔丁基甲醚。根据化合物的酸碱性,在提取的时候加缓冲液调解pH值,以提高回收率) 4,固相萃取(适合大部分化合物,成本比较高) 5,在线处理(发文章时常用,实际应用较少)
062250 2007/04/19
为什么没有好的回复啊?
xhongxue 2007/04/23
[quote]原文由 [B]maolg[/B] 发表: 一个很好的适合液质、[url=https://insevent.instrument.com.cn/t/bp][color=#3333ff]气质[/color][/url]的前处理方法 [img]https://www.instrument.com.cn/bbs/images/affix.gif[/img][url=http://www.instrument.com.cn/bbs/download.asp?ID=49070]ppt[/url][/quote] 大家可以看看 我简单介绍一下它的原理 干燥剂+样品溶液+萃取溶剂 一起震荡,离心取萃取溶剂进色谱分析 或 干燥剂+样品溶液+溶剂 一起震荡,离心,取溶剂用分散萃取剂再富集萃取一次后,进样 农业部将发布一种农药残留前处理新方法,做农药残留的可留意一下,是一种基于基质分散的原理,确十分方便,高效的方法,我从安捷伦工程师那询问得知,其做法与上述有相识之处
妮可 2007/04/30
我是作中药的, 新手一个, 接触液质时间不长, 我只说一点, 说的不对的或者不全面的, 还请各位大仙斧正: 中药我们前处理一般都是超声、回流、索氏、过柱子,萃取 然后在LC上分离,尽量找到最佳的色谱条件(分离度又好,保留时间又不长) 这就是我们的处理
瓢虫
第10楼2007/04/23
推荐一本书,"Mass Spectrometry in Drug Discovery",里面对液质联用有详细的介绍,从液质联用在药物研发中的应用,质谱的离子源,如何做液质联用,化合物在质谱中的断裂机理,样品前处理,液质联用在药代、组合化学、体外样品分析中的应用都有详细的介绍,这本书是几个大药厂的作者写的,很实用!
"Mass Spectrometry in Drug Discovery" edited by David T. Rossi & Michael W. Sinz (2002) ISBN 0-8247060-7-2.
This state-of-the-art reference summarizes the theory, instrumentation, and application of mass spectrometry and atmospheric pressure ionization to screen, evaluate, and improve the performance and quality of drug candidates梠ffering current techniques in pharmaceutics and pharmacokinetics for increased quantities of new pharmaceutical compounds. Providing time- and cost-efficient approaches for the generation and analysis of effective pharmaceuticals, Mass Spectrometry in Drug Discovery covers o recent advances in combinatorial chemistry, molecular biology, bioanalysis automation, and computing o cassette dosing in drug discovery o the utilization of liquid chromatography/mass spectrometry (LC/MS) in chemical library characterization, combinatorial chemistry, drug transport, and in vivo microdialysis o reliable methods of sample preparation and handling o novel strategies to determine and characterize metabolite structures o the impact of absorption, distribution, metabolism, and elimination properties on drug discovery o the latest research using stable-labeled compounds Demonstrating the use of mass spectrometry in the assessment of disease states, drug targets, and potential drug agents, Mass Spectrometry in Drug Discovery is an invaluable source for spectrometrists; pharmacologists; analytical, medicinal, and organic chemists and biochemists; biotechnologists; pharmaceutical scientists; toxicologists; and upper-level undergraduate and graduate students in these disciplines.
结合书上看的和个人的理解讲讲液质联用的前处理(主要针对药物方面的)。
主要的预处理方法有:
1,直接稀释(适合基质简单的样品,如制剂样品)
2,蛋白沉淀(适合极性较强的化合物,一般用甲醇或乙腈作为沉淀剂)
3,液液萃取(适合大部分化合物,可以选择一系列的提取试剂,极性强的一般选用:乙酸乙酯,非极性强的一般选用:叔丁基甲醚。根据化合物的酸碱性,在提取的时候加缓冲液调解pH值,以提高回收率)
4,固相萃取(适合大部分化合物,成本比较高)
5,在线处理(发文章时常用,实际应用较少)