【讨论】-关于农残检测中的基质效应的原理

附件中是一篇相关基质效应的综述文献，比较详细的介绍了国外对基质效应的解释及解决办法：
气相色谱分析农药残留的基质效应及其解决方法
贺利民,刘祥国,曾振灵
摘要:对于相同浓度的农药,其在基质溶液中的色谱响应会比其在纯溶剂中的响应高。通过减少热不稳定农药的分解,以及屏蔽进样口的活性位点而减少极性农药在活性位点的吸附或分解,基质效应可增加从进样口传输到色谱柱中的农药残留量。各种进样方式和基质净化方法都可以减少但不能完全消除基质效应；基质匹配校准法和分析保护剂法是避免基质效应最有效的方法;在实际检测中,所采用的消除或补偿基质效应的方法应考虑减少仪器系统的维护。本文概述了农药残留分析检测中的基质效应及其解决方法。
关键词:气相色谱;农药残留;基质效应;解决方法
[url=https://insevent.instrument.com.cn/t/Mp]气相色谱分析农药残留的基质效应及其解决方法[/url]

Explanation of the matrix-induced chromatographic response enhancement of organophosphorus pesticides during open tubular column gas chromatography with splitless or hot on-column injection and flame photometric detection
D.R. Erney, A.M. Gillespie and D.M. Gilvydis
ABSTRACT：
The observed chromatographic response for organophosphorus pesticides in extracts from milk and butterfat is shown to be matrix dependent. The matrix protects the organophosphorus compounds from adsorption and/or decomposition in hot vaporizing injectors ensuring a more complete transfer from injector to column compared to the results observed when standards dissolved in matrix-free solvent are used. This results in recoveries in excess of 180% for residue-free extracts spiked with organophosphorus pesticides when standards prepared in residue-free solvents are used for calibration. The chromatographic response enhancement is minimized by using hot on-column injection at an optimized injection temperature, but not completely eliminated. The preferred method of calibration is to use matrix standard solutions prepared by adding known amounts of organophosphorus pesticides to residue-free sample matrix of the same character and in similar concentration to the samples to be analyzed.
http://www.instrument.com.cn/bbs/download.asp?ID=135959

Matrix effects in (ultra)trace analysis of pesticide residues in food and biotic matrices
Jana Hajsˇlova´, Jitka Zrostl´ıkova
Abstract:
If one has to determine multiple residues of modern pesticides and/or other groups of (semi)polar and/or thermally
unstable contaminants with a wide range of physico-chemical properties within a single analytical run, it can be complicated to obtain low limits of quantitation (LOQ), good precision as well as relevant trueness of results for all the target analytes. Matrix components, which are unavoidably present in analyzed samples (even after the thorough clean-up step), may be responsible for miscellaneous adverse affects impairing different stages of the gc and/or $$lc determinative step. In this review, the nature of various types of matrix effects are discussed together with suggestions for prevention, reduction and/or compensation of their occurrence when determining troublesome analytes in foods and other complex biotic matrices.
http://www.instrument.com.cn/bbs/download.asp?ID=135958

以上几篇文献是关于基质效应的原理方面的文献，大家可以看看。（感谢dong3626版主提供英文全文！）

这两篇文献还是很不错的；我们做有机磷农药残留的时候经常会遇到基质效应。

应助达人

基质效应的原理搞不太清楚，但做农残开始，就一直在用，我做的物质，大多数是有了基质效应，峰面积是增加

那篇比较老了，这是介绍基质效应研究过程和现状比较全面的
Matrix-induced response enhancement in pesticide
residue analysis by gas chromatography
1
The sample matrix can cause an enhancement in the observed chromatographic response for pesticide residues in a matrix extract compared with the same concentration in a matrix-free solution. The matrix increases the transfer of pesticides from hot vaporizing injectors by reducing the thermal stress for labile compounds and by masking active sites in the injector responsible for the adsorption or decomposition of polar pesticides.
The use of different injector types and matrix simplification procedures can reduce matrix-induced enhancement but do not eliminate it. The most effective strategy is to use matrix-matched calibration standards or analyte protectants which equalize the response enhancement for calibration standards and sample extracts. From a practical point of view it is important that the method used to correct for matrix-induced enhancement is compatible with low system maintenance. The different approaches for correcting matrix-induced enhancement for calibration in pesticide residue analysis are discussed and compared in this review.

基质效应及其消除
[url=https://insevent.instrument.com.cn/t/Mp]气相色谱分析农药残留的基质效应及其解决方法[/url]
蔬菜农药残留GC_MS分析方法改进与基质效应探讨

希望这几个文献对大家有帮助

应助达人

10楼的一篇文章提到，减少进样量，也能消除基质效应，不知同仁们有没有试过，结果如何

《气相色谱分析农药残留的基质效应及其解决方法》
这篇文章就是翻译的下面这篇英文文章
《Matrix-induced response enhancement in pesticide
residue analysis by gas chromatography 》