【有奖问答】【离子色谱仪器故障之一】电导池如何校正？

积分拿来吧,我穷着呢,呵呵
1 进入Chromeleon的Wellness 面版,在External Conductivity Cell CALIBRATION 中点击 INSTRUCTION,了解操作过程.
2 将泵的出口与电导池的入口用至少1000PSI的反压管连接.
3 在EXTERNAL CONDUCTIVITY CELL CALIBRATION 中点击CELL35C的命令,等待电导池升温稳定至少5MIN.
4 以1.0ML/MIN 的流速泵送1MM KCL 校正溶液.
5 等待电导值稳定后(至少15MIN)执行CALIBRATE 命令
6 电导值应为147正负2us.
7 点击LOG,储存新的校正值.
8 用去离子水清洗电导池至电导读数接近于零后停泵.
9 将管路恢复原状.

回答完毕,给钱吧,呵呵祝好.

Calibrating the Conductivity Cell
When to Calibrate
• After every 6 months of use

NOTE Do not use this procedure to calibrate a new cell.

1. Open the ICS-1000 Wellness Panel in Chromeleon
2. On the Wellness Panel under Electric Conductivity Cell
Calibration, click Offset Cal.
3. When the offset calibration is complete, click Slope Cal on the
Wellness Panel.
4. Disconnect the pump output line from the injection valve.
5. Disconnect the line from the suppressor ELUENT OUT port to the cell
inlet and connect the pump output line directly to the cell inlet.
6. Verify that there is a minimum of 7 MPa (1000 psi) of backpressure.
7. On the Wellness Panel under Conductivity Cell Calibration, click
Cell 35 C. Allow the cell to reach this temperature and then wait an
additional 5 minutes to let it stabilize.
8. Select 1.00 mL/min to begin pumping 1.0 mM KCl through the cell at
1.0 mL/min.
9. Wait until the total conductivity reading stabilizes (in about
15 minutes) and then select Calibrate on the Wellness Panel.
After calibration, the conductivity reading should be 147.00 ± 2 μS
and the cell constant should be between 120 and 180. If this is not the case, contact Dionex for help.
10. Click Log to record the new calibration values in the Audit Trail.
11. Flush the KCl solution from the system by pumping deionized water
through the cell. When the conductivity drops to less than 1 μS/cm,
stop the pump.
12. Reconnect the pump to the injection valve and reconnect the line
from the suppressor to the cell inlet.

Items Needed Description
1.0 mM KCl solution ---- Prepare by dissolving 0.07456 g of reagentgrade
KCl in 1 liter of 18-megohm DI water.
Backpressure tubing to provide at least 7 MPa (1000 psi)-----Use 0.076-mm (0.003-in) ID yellow PEEK tubing (P/N 049715).



Thanks ！But chinese is easy to read ！

1、2楼都答得很充分了，我就不重复了。但不同的仪器校正方法有些差异，操作时要注意。
另外引发了一个思考：之前听戴安的工程师说过，电导池做校正其实没大必要。因为标样和样品都是在相同条件下走出来的，系统误差相同。另外，你即使做了校正，不同的人不同的条件也不一定准确！所以建议一般不做校正，如遇到较大误差，或怀疑电导池污染，清洗电导池后可做校正。

从1，2楼的校正步骤来看，个人觉得容易引起误差的主要是1.0mM KCl的配制问题上，因为那个关系到电导池读数的问题，其余地方应该问题不大，都是等稳定了才读数校正的。

以上问题由离子色谱群（1群8322724，2群81052181）成员H3BO3在群中提出，版主帮忙发帖在这里，多谢版主