高效液相色谱测量聚乙二醇200使用什么流动相、色柱？

应助达人

楼主测的是纯品还是混合物，请把样品的性质说清楚。

建议先查文献，这类物质做的很多，应该有相应的分析方法，先查查最保险

聚乙二醇药典的标准不是用气相做的吗？

参考
Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster, Ashley Elliott, Arik Dahan and Jonathan M. Miller

Anal. Methods, 2011, 3, 742-744


A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

我也记得聚乙二醇药典的标准是用气相