如题,本人研究生一枚,刚刚接触整体柱的合成这个方向,今天看到了一篇文献,具体如下: The prepared column was washed with ACN for 30 min and then perfused with 4% w/v DODCS in toluene while the column was maintained at 110 ◦C for 6 h (in a heated HPLC oven Model 851101 from DuPont, Wilmington, DE,USA) to yield a monolithic column 想问一下各位有能升到110摄氏度的柱温箱吗?(我只知道安捷伦1200的柱温最高60摄氏度)在网上没有找到关于Dupont 柱温箱的信息啊! 另外想问下大家对perfuse这个词的理解,是把反应液搁到流动相那个位置,然后用泵把反应液泵入整体柱反应吗??? 谢谢大家!!!