☜譕♥魚☞
第2楼2010/01/20
做蛋白的柱子压力都很高的
建议你
F lush with stronger solvents than your mobile phase
用比你的流动相更强的溶剂冲洗
Reversed-Phase Solvent Choices in Order of
Increasing Strength 为了增加强度选择反相溶剂
Use at least 25 ml of each solvent for analytical columns
对于分析柱每种溶剂至少用25 ml 冲洗
Mobile phase without buffer salts 没有缓冲盐的流动相
100% Methanol 甲醇
100% Acetonitrile 乙腈
75% Acetonitrile :25% Isopropanol 75乙腈 : 25%异丙醇
100% Isopropanol 异丙醇
100% Methylene Chloride★ 二氯甲烷
100% Hexane ★ 已烷
★ When using either Hexane or Methylene Chloride the column must be flushed with
Isopropanol before returning to your resvered-phase mobile phase.
当用已烷蔌二氯甲烷柱子时,必须先用异丙醇冲洗,然后再用你的反相流动相
hec_css
第4楼2010/01/21
如果我用8mol/L的脲冲洗,再用EDTA冲洗,再用水冲洗,再用乙腈冲洗,您看合理吗?