dongnan
第2楼2007/07/10
http://www.springerlink.com/content/f1t8v84162470876/
Summary An HPLC system has been developed in which 23 phenylthiocarbamyl (PTC) amino acids are distinctly separated. Applying this system, the preceding steps in HPLC amino acid analysis i.e. gas-phase hydrolysis of the protein/peptide sample and the pre-column derivatization, were evaluated and optimized. PTC amino acids in HPLC buffer solution are stable for at least 16 hours if stored at 5°–8°C. The sensitivity of the method is in the low pmol range.
Key Words Column liquid chromatography - Phenylthiocarbamyl amino acids - Pre-column derivatization - Gas-phase protein hydrolysis
可以在Springer-Link上面买到,但是需要国际信用卡。
实际上就是买一个密码,PDF文件可以随便下载。