【求助】求一段英文的翻译

Metabolites were extracted from the deep frozen cells by a pre-mixture of 300 µl
methanol (final concentration ~80 %), 30 µl nonadeconoic acid methylester (2 mg ml-1
chloroform) and 30 µl of ribitol (0.2 mg ml-1), and D4-2,3,3,3-alanine (1 mg ml-1, standards in methanol). Each sample was mixed thoroughly at least 1 min for complete suspension, agitated 15 min at 70°C, brought to room temperature mixed with 200 µl chloroform and agitated again 5 min at 37°C
用300µl甲醇（最终浓度约80%）， 30µl19酸甲酯（2 mg ml-1氯仿）和30µl核糖醇(0.2 mg ml-1), 和D4-2,3,3,3-alanine（alanine 丙氨酸，整个没找到）(1 mg ml-1,甲醇标液) 的预混物从深冻细胞提取代谢物。每个样品充分混合至少1分钟，以使样品完全悬浮，在70°C搅拌15分钟，冷却至室温，与200 µl氯仿混合，在37°C再搅拌5分钟。

啊谢谢！

我也主要是这个药物不知道是 个什么东东
呵呵
不过还是十分感谢您！